SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.
信号通路
Autophagy
靶点
Autophagy
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IC50
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体外研究
SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment.
体内研究
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临床实验
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特征
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相关实验数据(此数据来自于公开文献,信裕生物并不保证其有效性):
酶活性检测实验
方法
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细胞实验
细胞系
MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures
浓度
10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures
处理时间
16hrs
方法
MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.
动物实验
动物模型
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配制
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剂量
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给药方式
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参考文献: 1. Tian Y, et al. FASEB J. 2011, 25(6):1934-1942.
包装清单: