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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
Acetolactate Synthase (Crude Enzyme)
- 保质期:
1个月
- 供应商:
上海信裕生物科技有限公司
- 保存条件:
-20℃或更低温度保存,至少1个月有效。
- 规格:
100ml;1L
Acetolactate Synthase,中文名为乙酰乳酸合成酶,催化一分子酸脱羧并与另一分酸缩合成一分子乙酰乳酸,从而导致侧链氨基酸缬氨酸、亮氨酸和异亮氨酸的生物合成。本产品为大肠杆菌表达的乙酰乳酸合成酶,并经简单纯化获得的可以确保达到一定酶活力的粗酶液。
由于本产品需新鲜制备,确认订购后约6个工作日才可以发货。个别情况首次制备出现问题,发货时间会顺延。
本产品可以提供十公斤、百公斤乃至吨级的粗酶液,如有需求请联系信裕生物。
The Acetolactate Synthase (ALS) enzyme (also known as acetohydroxy acid synthase, or AHAS) is a protein found in plants and micro-organisms. ALS catalyzes the first step in the synthesis of the branched-chain amino acids (valine, leucine, and isoleucine). It is a human protein of yet unknown function, sharing some sequence similarity with bacterial ALS, and is encoded by the ILVBL gene. This product with the indicated enzyme activity was briefly purified from engineered E.coli.
产品详细信息见下表:
| About this product | |
| Name | Acetolactate Synthase (Crude Enzyme);乙酰乳酸合成酶(粗酶液) |
| Application | 催化一分子酸脱羧并与另一分子酸缩合成一分子乙酰乳酸,从而导致侧链氨基酸缬氨酸、亮氨酸和异亮氨酸的生物合成。 |
| Appearance | Clear to translucent yellow solution |
| Enzyme activity | Undetermined |
| About this enzyme | |
| Name | Acetolactate Synthase;乙酰乳酸合成酶 |
| CAS Number | 9027-45-6 |
| EC Number | 2.2.1.6 |
| Enzymatic Reaction | 2 pyruvate = 2-acetolactate + CO2 |
| Synonyms | α-acetohydroxy acid synthetase; α-acetohydroxyacid synthase; α-acetolactate synthase; α-acetolactate synthetase; acetohydroxy acid synthetase; acetohydroxyacid synthase; acetolactate pyruvate-lyase (carboxylating); acetolactic synthetase |
| Systematic name | pyruvate:pyruvate acetaldehydetransferase (decarboxylating) |
| Cofactor(s) | FAD; thiamine diphosphate |
| Application | agriculture; biotechnology; drug development; synthesis; molecular biology |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| XY-3609-100ml | Acetolactate Synthase (Crude Enzyme) | 100ml |
| XY-3609-1L | Acetolactate Synthase (Crude Enzyme) | 1L |
| — | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存,至少1个月有效。
注意事项:
由于本产品需新鲜制备,在您确认订购后约6个工作日才能发货。个别情况首次制备出现问题,发货时间会顺延。
粗酶液的每次冻融均可能会引起部分失活,所以收到酶液后请根据需要进行分装,并置于-20℃或更低温度保存。
随着保存时间的延长,酶活力会有一定程度的下降,收到产品后应尽快使用。
本产品在生产和保存的过程中可能会有混浊或沉淀产生,融化后混匀即可,不影响正常使用。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明:
1.收到产品后请立即使用或按照说明书推荐的条件保存。随着保存时间的延长,酶活力会有一定程度的下降。
2.具体的最佳工作条件请自行参考相关文献,或者通过实验进行摸索和优化。
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文献和实验Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated
Measurement of NO and NO Synthase
Oxide Synthase (NOS) Activity Support Protocol 4: Preparation and Regeneration of Cation‐Exchange Columns Support Protocol 5: Purification of Radiolabeled L‐Arginine Support Protocol
Assay of Deoxyhypusine Synthase Activity
Deoxyhypusine synthase catalyzes an unusual protein modification reaction. A portion of spermidine is covalently added to one specific lysine residue of one eukaryotic protein, eIF5A (eukaryotic initiation factor 5A) to form a deoxyhypusine
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