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- TA500465S
- 2026年04月23日
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- 技术资料
- 规格：
30 ul
predicted_size：47.2 kDa
Uniprot ID：Q14653
description：Anti-IRF3 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2)
clonality：Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...)：Primary Antibodies
background：IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
immunogen：Full-length protein expressed in 293T cell transfected with human IRF3 expression vector
recommended_dilution：WB 1:2000 IHC 1:50
predicted_size：47.2 kDa
buffer：PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species：Mouse
applications：IHC, WB
reactivities：Human, Monkey
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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
-4326) or cyclin D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20°C) cyclin
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20℃) cyclin staining
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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