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30 ul
predicted_size:48.1 kDa
Uniprot ID:O686
description:TBX19 (Tpit) mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)
clonality:Monoclonal
product_type(primary antibody, secondy antibody ,tag antibody,Loding Control...):Primary Antibodies
background:This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Mutations in this gene were found in patients with isolated deficiency of pituitary POMC-derived ACTH, suggesting an essential role for this gene in differentiation of the pituitary POMC lineage. ACTH deficiency is characterized by adrenal insufficiency symptoms such as weight loss, lack of appetite (anorexia), weakness, nausea, vomiting, and low blood pressure. [provided by RefSeq, Jul 2008]
immunogen:Human recombinant protein fragment corresponding to amino acids 1-238 of human TBX19 (NP_005140) produced in E.coli.
recommended_dilution:WB 1:2000
predicted_size:48.1 kDa
buffer:PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
host_species:Mouse
applications:WB
reactivities:Human, Mouse
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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