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文献和实验(only needed for elution). PBS pH 7.4 (Phosphate Buffered Saline - for buffy coat, see below). Sterile and PCR-grade water for dilution of supplied buffers. 实验步骤 1. DNA Isolation Protocol from 100 μl Blood 1)
, temperatures, and amount of template DNA, may vary and must be individually determined. 1) Thaw primer solutions. Keep on ice after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important
after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important to mix the PCR Master Mix before use to avoid localized differences in salt concentration. 3) Prepare one of the following reaction
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