- ￥888 - 1169
- 碧云天（beyotime）
- D2259
- 2025年08月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 规格：
1μg/100μg
| 规格： | 1μg | 产品价格： | ￥888.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1169.0 |
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D2259-1μg | pSTAT3-TA-luc (报告基因质粒) | 1μg | 888.00元 |
| D2259-100μg | pSTAT3-TA-luc (报告基因质粒) | 100μg | 1169.00元 |
pSTAT3-TA-luc (报告基因质粒)是碧云天自行研发的用于检测STAT3(Signal transducer and activator of transcription 3)转录活性水平的报告基因质粒。pSTAT3-TA-luc是以碧云天的pGL6-TA质粒为模板，在其多克隆位点插入了多个STAT3结合位点，可以高灵敏度地检测STAT3的激活水平。
pGL6-TA质粒是用于在哺乳动物细胞中进行萤火虫萤光素酶(firefly luciferase)报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进，一方面对于luciferase的编码进行了改进，确保能更好地在哺乳动物细胞中进行表达，同时对整个质粒中所有可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理，在保持原有功能不变的情况下，使各种转录因子在质粒上的非特异性结合降到最低。
萤光素、萤光素酶、萤火虫萤光素酶和海肾萤光素酶也经常被称作荧光素、荧光素酶、萤火虫荧光素酶和海肾荧光素酶。
pSTAT3-TA-luc质粒的主要信息如下：
| Base pairs | 5672 |
| STAT3 response element | 32-86 |
| Minimal TA promoter (pTA) | 109-131 |
| luc2 reporter gene | 173-1825 |
| SV40 late poly(A) signal | 1860-2081 |
| SV40 early enhancer/promoter | 2129-2547 |
| Synthetic neomycin phosphotransferase | |
| (Neor) coding region | 2572-3366 |
| Synthetic poly(A) signal | 3391-3439 |
| Reporter Vector primer 4 (RVprimer4) binding region | 3506-3525 |
| ColE1-derived plasmid replication origin | 3763 |
| Synthetic Beta-lactamase (Ampr) coding region | 4554-5414 |
| Synthetic poly(A) signal/transcriptional pause site | 5519-5672 |
| Reporter Vector primer 3 (RVprimer3) binding region | 5621-5640 |
pSTAT3-TA-luc质粒的图谱如下：
pSTAT3-TA-luc的多克隆位点及STAT3 response element的详细图谱见说明书。
pSTAT3-TA-luc中没有的酶切位点(Restriction enzymes that do not cut pSTAT3-TA-luc)包括：
| Aat II | Afl II | Asc I | Ase I | Bsa I | BsaA I | BsiW I | BspM II |
| BssH II | Eco72 I | EcoR I | EcoR V | Mlu I | Nde I | Nru I | PflM I |
| Pme I | Pml I | PspA I | Rsr II | Sac I | Sma I | SnaB I | Spl I |
| Srf I | Tth111 I | Vsp I | Xcm I | Xma I | Xmn I |
pSTAT3-TA-luc中的单酶切位点(Restriction enzymes that cut pSTAT3-TA-luc once)包括：
| Sfi I | GGCCN,NNN`NGGCC | 9 | BsiC I | TT`CG,AA | 3442 | |
| Bgl I | GCCN,NNN`NGGC | 9 | BstB I | TT`CG,AA | 3442 | |
| Acc65 I | G`GTAC,C | 15 | Sal I | G`TCGA,C | 3456 | |
| Asp718 | G`GTAC,C | 15 | Afl III | A`CRYG,T | 3706 | |
| Kpn I | G,GTAC`C | 19 | ApaL I | G`TGCA,C | 4020 | |
| Nhe I | G`CTAG,C | 21 | HgiE II | ACCNNNNNNGGT -1/13 | 4285 | |
| PaeR7 I | C`TCGA,G | 27 | Not I | GC`GGCC,GC | 4526 | |
| Xho I | C`TCGA,G | 27 | BstX I | CCAN,NNNN`NTGG | 4550 | |
| Bgl II | A`GATC,T | 88 | BstE II | G`GTNAC,C | 4553 | |
| Hind III | A`AGCT,T | 98 | Ahd I | GACNN,N`NNGTC | 4628 | |
| BsrG I | T`GTAC,A | 664 | Bsu36 I | CC`TNA,GG | 4984 | |
| Dra III | CAC,NNN`GTG | 1320 | Pvu I | CG,AT`CG | 4998 | |
| Gsu I | CTGGAG 21/19 | 1553 | Sac II | CC,GC`GG | 5022 | |
| Bpm I | CTGGAG 22/20 | 1554 | Bst1107 I | GTA|TAC | 5138 | |
| Apo I | R`AATT,Y | 1936 | Xca I | GTA|TAC | 5138 | |
| Mun I | C`AATT,G | 2000 | Spe I | A`CTAG,T | 5457 | |
| BamH I | G`GATC,C | 2093 | BsmA I | GTCTC`/9 | 5469 | |
| Stu I | AGG|CCT | 2526 | BsmB I | CGTCTC 7/11 | 5470 | |
| EcoN I | CCTNN`N,NNAGG | 3047 |
pSTAT3-TA-luc质粒中推荐使用的测序引物序列如下：
RVprimer3 (5621-5640):
CTA GCA AAA TAG GCT GTC CC
pSTAT3-TA-luc的全序列信息： D2259 pSTAT3-TA-luc-seq.txt
包装清单：
| 产品编号 | 产品名称 | 包装 |
| D2259-1μg | pSTAT3-TA-luc (报告基因质粒) | 1μg |
| D2259-100μg | pSTAT3-TA-luc (报告基因质粒) | 100μg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存。
注意事项：
本质粒未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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- 作者
- 内容
- 询问日期
文献和实验1. Liao Q, Zeng Z, Guo X, Li X, Wei F, Zhang W, Li X, Chen P, Liang F, Xiang B, Ma J, Wu M, Tang H, Deng M, Zeng X, Tang K, Xiong W, Li G. . LPLUNC1 suppresses IL-6-induced nasopharyngeal carcinoma cell proliferation via inhibitingthe Stat3 activation. Oncogene. 2014 Apr 17;33(16):2098-109. (IF 7.971)
2. Zha X, Hu Z, He S, Wang F, Shen H, Zhang H. . TSC1/TSC2 inactivation inhibits AKT through mTORC1-dependent up-regulation ofmSTAT3-PTENcascade. Cancer Lett. 2011 Dec 27;313(2):211-7. (IF 7.36)
3. Liu LJ, Leung KH, Chan DS, Wang YT, Ma DL, Leung CH. . Identification of a natural product-like STAT3 dimerization inhibitor by structure-based virtual screening. Cell Death Dis. 2014 Jun 12;5:e1293. (IF 6.304)
4. Wang S, Yao Y, Wang X, Zheng G, Ouyang W, Chen W . 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4. Am J Cancer Res. 2019 Oct 1 9(10):2140-2155. eCollection 2019. (IF 5.177)
5. Huang Y, Yang M, Hu H, Zhao X, Bao L, Huang D, Song L, Li Y. . Mitochondrial GRIM-19 as a potential therapeutic target for STAT3-dependent carcinogenesis of gastriccancer. ONCOTARGET. 2016 Jul 5;7(27):41404-20. (IF 5.168)
6. Chen X, Du J, Zhu Y, Zhang C, Sun H . Comprehensive analysis of lncRNA and mRNA expression profiles in macrophages activated by Actinidia eriantha polysaccharide. Int J Biol Macromol. 2019 Sep 1 136:980-993. (IF 5.162)
7. Song ZB, Bao YL, Zhang Y, Mi XG, Wu P, Wu Y, Yu CL, Sun Y, Zheng LH, Huang YX, Liu B, Li YX. . Testes-specific protease 50 (TSP50) promotes cell proliferation through the activation of thenuclear factor κB (NF-κB) signalling pathway. Biochem J. 2011 Jun 1;436(2):457-67. (IF 4.097)
8. Han L, Yue X, Zhou X, Lan FM, You G, Zhang W, Zhang KL, Zhang CZ, Cheng JQ, Yu SZ, Pu PY, Jiang T, Kang CS. . MicroRNA-21 Expression is regulated by β-catenin/STAT3 Pathway and Promotes GliomaCellInvasion by Direct Targeting RECK. CNS Neurosci Ther. 2012 Jul;18(7):573-83. (IF 4.074)
9. Wei H, Wang X, Zhang A, Du L, Zhou H. . Identification of grass carp IL-10 receptor subunits: functional evidence for IL-10 signaling in teleost immunity. Dev Comp Immunol. 2014 Aug;45(2):259-68. (IF 3.192)
10. Zhao Y, Yao J, Wu XP, Zhao L, Zhou YX, Zhang Y, You QD, Guo QL, Lu N. . Wogonin suppresses human alveolar adenocarcinoma cell A549 migration in inflammatory microenvironment by modulating the IL-6/STAT3 signaling pathway. MOL CARCINOGEN. 2015 Jun;54 Suppl 1:e81-93. (IF 3.825)
11. Kong L, Li S, Yu X, Fang X, Xu A, Huang M, Wu X, Guo Y, Guo F, Xu J. . Hepatitis C virus and its protein NS4B activate the cancer-related STAT3 pathway via the endoplasmicreticulum overload response. Arch Virol. 2016 Aug;161(8):2149-59. (IF 2.243)
12. Ai HH, Zhou ZL, Sun LG, Yang MT, Li W, Yu CL, Song ZB, Huang YX, Wu Y, Liu L, Yang XG, Zhao YQ, Bao YL, Li YX. . 20(S)-25-methoxyl-dammarane-3β, 12β, 20-triol negatively regulates activation of STAT3 and ERK pathways and exhibits anti-cancer effects in HepG2 cells. Apoptosis. 2017 Nov;22(11):1404-1418. (IF 4.543)
13. Zhang ZL, Jiang QC, Wang SR. . Schisandrin A reverses doxorubicin-resistant human breast cancer cell line by the inhibition of P65 and Stat3 phosphorylation. Breast Cancer. 2018 Mar;25(2):233-242. (IF 2.044)
14. Fan XY, He D, Sheng CB, Wang B, Wang LJ, Wu XQ, Xu L, Jiang JL, Li L, Chen ZN . Therapeutic anti-CD147 antibody sensitizes cells to chemoradiotherapy via targeting pancreatic cancer stem cells. Am J Transl Res. 2019 Jun 15 11(6):3543-3554. eCollection 2019. (IF 3.375)
15. Huihan Ai, Hongshuang Qin, Jiawei Li, Chunxue Niu, Zhenbo Song, Yongli Bao, Luguo Sun, Lihua Zheng, Yuxin Li . Ethyl-p-methoxycinnamate enhances oct4 expression and reinforces pluripotency through the NF-κB signaling pathway Biochem Pharmacol. 2020 Jul;177:113984. doi: 10.1016/j.bcp.2020.113984. (IF 4.96)
16. Tao Pan, Fang Zhang, Fakai Li, Xingchun Gao, Zhikui Li, Xia Li, Xinling Ren . Shikonin blocks human lung adenocarcinoma cell migration and invasion in the inflammatory microenvironment via the IL‑6/STAT3 signaling pathway Oncol Rep. 2020 Sep;44(3):1049-1063. doi: 10.3892/or.2020.7683. (IF 3.417)
17. Yuanzhu Xie, Shuaiwen Zhu, Ling Chen, Hongdou Liu, Ting Peng, Zhengnan Ming, Zizheng Zou, Xiyuan Hu, Wensong Luo, Kunjian Peng, Yuan Nie, Tiao Luo, Dayou Ma, Suyou Liu, Zhiyong Luo . An Isoxazoloquinone Derivative Inhibits Tumor Growth by Targeting STAT3 and Triggering Its Ubiquitin-Dependent Degradation Cancers (Basel). 2023 Apr 23;15(9):2424. doi: 10.3390/cancers15092424.
