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20μg
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 244332 | Q7TNV9 | E. coli | 45aa | 5.2kDa | － |
| About this Protein | |
| Name | Recombinant Murine BD-14 (Recombinant Murine Beta-defensin 14; rMuBD-14)；Recombinant Mouse BD-14 ; 重组小鼠β-防御素14 |
| Synonyms | BD14 |
| Purity | >96% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Testing in progress. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 0.1EU/μg of rMuBD-14 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. |
| Amino Acid Sequence | FLPKTLRKFF CRIRGGRCAV LNCLGKEEQI GRCSNSGRKC CRKKK |
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