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- 规格:
2μg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P5579-2μg | Recombinant Human PDGF-BB | 2μg | 646.00元 |
| P5579-10μg | Recombinant Human PDGF-BB | 10μg | 1613.00元 |
| P5579-100μg | Recombinant Human PDGF-BB | 100μg | 7709.00元 |
| P5579-1mg | Recombinant Human PDGF-BB | 1mg | 24657.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 5155 | P01127 | E. coli | 110aa | 24.8kDa | - |
| About this Antibody | |
| Name | Recombinant Human PDGF-BB (Recombinant Human Platelet-derived Growth Factor-BB; rHuPDGF-BB);重组人血小板衍生生长因子-BB |
| Synonyms | Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Pdgfb |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3ng/ml, corresponding to a specific activity of >3.3×105IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | Less than 1EU/μg of rHuPDGF-BB as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |
| Amino Acid Sequence | MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5579-2μg | Recombinant Human PDGF-BB | 2μg |
| P5579-10μg | Recombinant Human PDGF-BB | 10μg |
| P5579-100μg | Recombinant Human PDGF-BB | 100μg |
| P5579-1mg | Recombinant Human PDGF-BB | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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