Frontiers in pharmacology 2019 - FIGURE 2 Expression of PD-L1 on human HCC PLC/PRF/5 cells. (A) Human HCC PLC/PRF/5 cells were cultured alone in the absence of GPC3-CAR T cells in RPMI 1640 medium containing 10% FBS. (B) Human HCC PLC/PRF/5 cells were cocultured with GPC3-CAR T cells at an effector:Target ratio of 1:1 for 18 h in RPMI 1640 medium containing 10% FBS. PD-L1 was determined by flow cytometry in the CD3-negative gate, and... View more 查看全部结果图片 点击以上链接,即可跳转到BenchSci网页。免责声明
Frontiers in pharmacology 2019 - FIGURE 4 Efficient disruption of PD-1 expression on the surface of GPC3-CAR T cells. PD-1 and CAR expression on the surface of T cells were detected by flow cytometry on day 3 after the re-stimulation with anti-CD3/anti-CD28 beads. UTD, untransduced T cells; WT, wild type.
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Scientific reports 2018 - Figure 1 In vitro T cell proliferation. Mononuclear cells from psoriatic patients (red curve) and healthy controls (blue curve) were labelled with 1 uM CFSE prior to culturing and incubated for 5 days alone, with 1 ug/ml PHA ( A ) or in the presence of recombinant proteins K17 ( B ), S1 ( C ), S4 ( D ), PS1 ( E ) and PK ( F ) at concentration of 10 ug/ml. After 5 days, cells were labelled with a PE-conjugat... View more 查看全部结果图片 点击以上链接,即可跳转到BenchSci网页。免责声明
PloS one 2017 - Figure 7 Circulating percentages of Th17 cells and CCR6-positive cells in peripheral blood are increased in IVD degenerated patients when compared with controls. Heparinized peripheral whole blood cells from 20 patients and 15 healthy controls were stimulated with phorbol myristate acetate (PMA), ionomycin, and monensin for 4 h and subsequently stained with fluorochrome-labeled antibodies as described in Ma... View more 查看全部结果图片 点击以上链接,即可跳转到BenchSci网页。免责声明
Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD3 FITC (right). Cells in the lymphocyte gate were used for analysis.
Relative expression
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
CD3 Antibody (11-0036-42)
Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone SK7 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone SK7 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone SK7) and CD19 (clo... View more