Invitrogen CD3e Monoclonal Ant

Invitrogen CD3e Monoclonal Antibody (145-2C11), FITC, eBioscience™ 11-0031-63 ，11-0031-81， 11-0031-82， 11-0031-85 ，11-0031-86

数据图库

• 已发表图片 (40)
• 抗体检测数据 (2)

数据图库

• 已发表图片 (40)
• 抗体检测数据 (2)

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FIGURE 2/42

CD3e Antibody (11-0031-63)

Scientific reports 2018 - Figure 2 Characterization of spleens from WT and Ats1-KO mice. (a) Graph representing the spleen index (as indicated in the Methods section) of healthy WT (n = 6) and Ats1-KO (n = 5) mice to assess splenomegaly. Inset below include representative images of spleens from WT and Ats1-KO mice (white scale bar = 1 cm). (b) Graph representing flow cytometry data, as percentage of positive cells of the followi... View more
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FIGURE 1/42

CD3e Antibody (11-0031-63)

eLife 2018 - Published figure using CD3e monoclonal antibody (Product # 11-0031-63)
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FIGURE 3/42

CD3e Antibody (11-0031-63)

Scientific reports 2018 - Figure 3 Characterization of bone marrow from WT and Ats1-KO mice. (a) Graph representing flow cytometry data, as percentage of positive cells of the following populations: CD45R + , CD3 + , CD11b + , GR1 + , CD11b + /GR1 + , and F4/80 + , found in bone marrow of healthy WT (n = 5) and Ats1-KO (n = 5) mice. (b) Graph representing the mRNA fold change expression of Cd3g , Il12a , Cd11b , Cd163 and No...View more
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FIGURE 4/42

CD3e Antibody (11-0031-63)

Scientific reports 2018 - Figure 4 Characterization of immune infiltration, versican and versicanases in B16F1 tumors in WT and Ats1-KO mice. (a) Graph representing flow cytometry data, as percentage of positive cells of the following populations: CD45R + , CD3 + , CD11b + , CD11b + /GR1 + , and F4/80 + , found in tumours of WT (n = 5) and Ats1-KO (n = 5) mice. (b) Graph representing the relative mRNA fold change expression of ... View more
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FIGURE 5/42

CD3e Antibody (11-0031-63)

Scientific reports 2018 - Figure 5 Characterization of spleens from B16F1 tumour-bearing WT and Ats1-KO mice. (a) Graph representing spleen index (as indicated in Methods section) of healthy and tumour-bearing mice (WT, n = 6; Ats1-KO, n = 5) to assess splenomegaly. (b) Graph representing flow cytometry data, as percentage of positive cells of the following populations: CD45R + , CD3 + , CD11b + , CD11b + /GR1 + , and F4/80 + , ... View more
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• 已发表图片 (40)
• 抗体检测数据 (2)

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FIGURE 7/42

CD3e Antibody (11-0031-63)

Neoplasia (New York, N.Y.) 2018 - Figure 7 R-Tf-D-LP4 treatment of DEN-induced HCC eliminates hepatosteatotic pathology, inflammation, and fibrosis. (A-E) Representative liver sections from DEN-treated, R-Tf-D-LP4-untreated, or R-Tf-D-LP4-treated mice stained with Sirius red (A) or anti-alpha-SMA (B) or anti-F4/80 antibodies (C) showing macrophage infiltration. IF staining for CD3+ and B220 (D), CD4+, CD8+, or FOXP3 (E). The relative staini... View more
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产品细节

已验证应用
稀释倍数
流式细胞分析 (Flow)
0.5 µg/test
免疫细胞化学 (ICC)
10 µg/mL
免疫荧光 (IF)
10 µg/mL
免疫组化（冰冻） (IHC (F))
10 µg/mL
已发表应用
流式细胞分析 (Flow)
查看下方已发表的148篇文献
免疫荧光 (IF)
查看下方已发表的7篇文献
T细胞活化 (TCA)
查看下方已发表的4篇文献
免疫组化 (IHC)
查看下方已发表的3篇文献
免疫印迹 (WB)
查看下方已发表的1篇文献
免疫沉淀 (IP)
查看下方已发表的1篇文献
其他PubMed (Misc)
查看下方已发表的1篇文献
免疫组化（冰冻） (IHC (F))
查看下方已发表的2篇文献
功能检测 (FN)
查看下方已发表的2篇文献
产品规格
种属反应
Mouse
已发表种属
Human, Mouse
宿主/亚型
Armenian hamster / IgG
推荐的同型对照
Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™
分类
Monoclonal
类型
Antibody
克隆号
145-2C11
偶联物
FITC
查看其它类型 
激发/发射光谱
查看光谱 
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.1% gelatin
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
RRID
AB_464880

产品详细信息

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 145-2C11 antibody has been tested by immunohistochemistry on frozen mouse spleen and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。
 

生物信息学

蛋白别名: CD3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain

基因别名: AI504783; CD3; Cd3e; CD3epsilon; T3e

UniProt ID: (Mouse) P22646

Entrez Gene ID: (Mouse) 12501

分子生物学功能:  cytokine receptor  defense/immunity protein  immunoglobulin receptor superfamily  receptor