Sample:
Testis (Rat) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-ALKBH5 (bs-20540R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35-44 kD
Observed band size: 40 kD
- ￥1180 - 2800
- Bioss
- bs-20540R
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20540R |
| 英文名称 | ALKBH5 Rabbit pAb |
| 中文名称 | ABH5蛋白抗体 |
| 英文别名 | ABH5; AlkB, alkylation repair homolog 5(E. coli); AlkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ALKBH5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > DNA Damage Response |
| 亚基 | Monomer. |
| 亚细胞定位 | Nucleus speckle |
| 相似性 | Belongs to the alkB family. |
| 功能 | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A)RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export and is required for spermatogenesis. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 6.1 }}] {Nan Zhang. et al.RNA m6A involves in regulation of oxidative stress and apoptosis may via NF-kB pathway in cadmium-induced lung cells.CELL DEATH DISCOVERY.2025 Jan 10;11(1):4.} {Western blot} {}
[IF={{ 3.2 }}] {Yuxin Liu. et al. N6-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks. LIFE-BASEL. 2024 Sep;14(9):1155} {IHC,WB,IF} {Bovine}
[IF={{ 3.2 }}] {Yuxin Liu. et al.N⁶-Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks.life.} {IF} {Yak, Bos taurus}
[IF={{ 2.9 }}] {Nan Zhang. et al. N6-methyladenosine mediated-NRF2 signaling pathway attenuates cadmium cytotoxicity by inhibiting oxidative damage in bronchial epithelial cells. TOXICOL LETT. 2025 Jan;403:144} {WB} {Human}
[IF={{ 2.7 }}] {Qiuyan Peng. et al. Enterovirus 71 structural viral protein 1 promotes the expression of PMP22 through m6A modification in mouse Schwann cells. VIRUS RES. 2025 Jun;:199590} {WB} {Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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ALKBH5 Rabbit pAb(bs-20540R)-50ul/100ul/200ul
￥1180 - 2800
