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- 2025年10月17日
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200ul
| 产品编号 | D50396s |
| 英文名称 | MG-132 solution (10mM/L) |
| 中文名称 | MG132溶液 (10mM/L) |
| 英文别名 | MG132; Z-LLL-CHO; Z-Leu-Leu-Leu-CHO; Z-Leu-Leu-Leu-al; Carbobenzoxy-L-leucyl-L-leucyl-L-leucinal; proteasome inhibitor; |
| 中文别名 | Proteasome抑制剂; 蛋白酶体抑制剂; |
| 保存条件 | Store at -80°C for 6 month. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MG-132 is a peptide aldehyde and a potent, reversible, and cell-permeable proteasome inhibitor with an IC50 of 100 nM, and effectively blocks the proteolytic activity of the 26S proteasome complex. |
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文献和实验[IF={{ 14.1 }}] {Yu Zhang. et al. Ezetimibe Engineered L14-8 Suppresses Advanced Prostate Cancer by Activating PLK1/TP53-SAT1-Induced Ferroptosis. ADV SCI. 2025 Jun;:e04192} {} {}
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
, 1 mM ATP, 2 mM DTT, 1 mM MgCl2 , 2 mg/ml Tween-20, 7.5% (w/v) Triton X-100 and 0.2 mM PMSF) and stir gently at 30°C for 30 min. The extract was dialyzed against Buffer D (10 mM Tris-Cl, pH 8.0, 0.2 mM CaCl2 , 0.5 mM ATP, 0.1 mM dithiothreitol (DTT), 100
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
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