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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃避光保存,有效期12个月。
- 英文名:
1 x PBS, calcium and magnesium, sterile
- 库存:
999
- 供应商:
北京博奥森生物技术有限公司
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
passaging 实验设备 Cell Scraper Dulbecco’s PBS (DPBS) with Calcium and Magnesium Dulbecco’s PBS (DPBS) without Calcium and Magnesium Geltrex® LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix
Differentiating Neural Stem Cells into Neurons and Glial Cells
步骤 1. Preparing Matrix 1) Coating Culture Vessels with CELLstart™ a. Dilute CELLStart™ CTS® 1:100 in D-PBS with calcium and magnesium (i.e., 50 μL of CELLStart™ CTS® into 5 mL of D-PBS). b. Coat the surface
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