相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃避光保存,有效期12个月。
- 英文名:
Sucrose-PBS solution (30%)
- 库存:
999
- 供应商:
北京博奥森生物技术有限公司
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Sucrose Fractionation of Castor Bean
Materials Castor beans Gradient former Sucrose solutions in 0.01 M EDTA, pH 7.5 33%, 44%, 50%, 57%, 60% (w/v) Grinding Medium 0.4 M sucrose 0.165 M Tris-HCl buffer, pH 7.5 0.01 M KCl
到7.4 然后加水补充到1000ml,15磅20分高压灭菌 或者滤膜除菌 室温保存免疫学手册上的配方:0.23 g NaH2PO4(anhydrous:1.9mM)1.15 g Na2HPO4(anhydrous:8.1mM)9.00g NaCl(154mM)加水 900 ml 调pH 7.2--7.4 再加水补充到1LPBS的配方:5X PBS stock solution80 gm NaCl2 gm KCl21.72 gm Na2HPO4-7H2O2 gm KH2PO4add to total
Sucrose Density Gradient Fractionation
at 4o until dissolved (around an hour)Remove flea by taking a big stir bar and putting it against the outside of the tube,moving it upwards.Gently overlay with 1 ml cold sucrose solutions of 60%,50%,40%,30%Balance tubes with 30% sucrose solution (weigh
技术资料暂无技术资料 索取技术资料







