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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Upon receipt, aliquot and store the Blocking Reagent and MDA-HDL Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
- 库存:
100
- 规格:
96 assays
2 parts: 1 RT / 1 Blue Ice
存储温度:
Upon receipt, aliquot and store the Blocking Reagent and MDA-HDL Standard at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
检测方法:
Colorimetric
类别:
Kit
产品分类:
Metabolism Research->Lipoprotein Metabolism->Oxidized HDL ELISA Kits
产品描述:
The OxiSelect™ Human Oxidized HDL ELISA Kits are enzyme immunoassays developed for the detection and quantitation of human oxHDL in plasma, serum or other biological fluid samples. This oxidized HDL assay kit is configured to selectively measure MDA-modified HDL. The kit contains an MDA-HDL standard against which unknown samples may be compared. Note: For samples stored for more than one month, better results may be obtained with measurement of CML or HNE modifications, both of which are more stable than MDA.
联系方式:
生物商城:http://www.annoron.com
办公电话:010-56256916
官方售后:400-965-8633
企业 QQ:3212874516、3198592576
地 址:北京经济技术开发区永昌中路4号院4号楼3层
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
analysis. For a comprehensive list of real-time thermal cyclers please see the weblink at the end of this article. Tools for Real-Time RT-PCR Ambion’s MessageSensor™ RT Kit includes an RNase H+ MMLV RT that clearly outperforms MMLV RT enzymes
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