S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit产品图

S-Adenosylmethionine (SAM) and

S-Adenosylhomocysteine (SAH) ELISA Combo Kit
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  • $13050
  • CELL BIOLABS已认证
  • STA-671-C
  • 2025年12月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Upon receipt, store the SAM Conjugate, SAH Conjugate, SAM-BSA Standard and SAH-BSA Standard at -80ºC. Store the rest of the kit at 4ºC.

    • 库存

      100

    • 规格

      96 assays

    运输温度:
    2 parts: 1 RT / 1 Blue Ice
    存储温度:
    Upon receipt, store the SAM Conjugate, SAH Conjugate, SAM-BSA Standard and SAH-BSA Standard at -80ºC. Store the rest of the kit at 4ºC.
    检测方法:
    Colorimetric
    类别:
    Kit
    产品分类:
    Metabolism Research->Amino Acid Metabolism->S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit
    产品描述:
    S-adenosylmethionine (SAM) is a substrate for methyl transfers to various biomolecules that yields S-adenosylhomocysteine (SAH). SAH is then hydrolyzed to homocysteine and adenosine, which is converted to methionine, and ultimately back to SAM. SAH can act as a methylation inhibitor, therefore the SAM/SAH ratio, or methylation potential, is important for maintaining proper methylation in the cell. Our S-Adenosylmethionine (SAM) and S-Adenosylhomocysteine (SAH) ELISA Combo Kit is designed for detection and quantitation of both SAM and SAH in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-SAM or anti-SAH antibody are added to a plate coated with a SAM or SAH conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high SAM and SAH levels will bind the majority of the antibody, which gets washed away and results in a low OD, while samples with low SAM and SAH levels will leave more antibody available to bind to the conjugate, producing a high signal.
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