- $7110
- CELL BIOLABS
- MET-5024
- 2025年12月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
- 库存：
100
- 规格：
100 assays
Dry Ice
存储温度：
Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
检测方法：
Fluorometric
类别：
Kit
产品分类：
Metabolism Research->Lipoprotein Metabolism->Phospholipid Assays
产品描述：
Our Phosphatidylglycerol/Cardiolipin Assay Kit measures both phosphatidylglycerol and cardiolipin in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipin levels in unknown sampled are determined based on the provided cardiolipin standard curve.
联系方式：
生物商城：http://www.annoron.com
办公电话：010-56256916
官方售后：400-965-8633
企业 QQ：3212874516、3198592576
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中，最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒（BCA Protein Assay Kit）。因为BCA法蛋白定量具有很多优点，如1.实验操作十分简单方便;2.实验耗时短，快速;3.试剂灵敏度高，实验结果准确;4.所用的试剂稳定性好，而且经济实用，抗干扰能力强等。 实验原理： BCA(bicinchoninic acid)法蛋白浓度定量
method. The Active Motif ChIP-IT Kit comes with all the reagents needed for 25 reactions of the ChIP assay. In addition, it provides a detailed manual which saves time for protocol optimization. The protocol in the manual is easy to follow
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