- ￥2617.94
- nsjbio
- F53890-0.05ML
- 2025年12月05日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A portion of amino acids 214-248 from the human protein was used as the immunogen for the RSPO3 antibody.
- 亚型：
Rabbit Ig
- 适应物种：
Human
- 宿主：
Rabbit
- 规格：
0.05 ml
Primary antibody
纯化方法：
Antigen affinity purified
通用信息：
In 1X PBS, pH 7.4, with 0.09% sodium azide
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
to agarose beads in chromatin samples prior to antibody reaction. (17) Pellet the agarose beads by brief centrifugation (3000-5000 x g (ex. 6600 rpm at Eppendorf table top centrifuge) for 1 min)). (18) Collect supernatants into new tubes. Remove
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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