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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.
- 亚型:
Mouse IgG1, kappa
- 克隆性:
单克隆
- 适应物种:
Human. Does not react with Mouse and Rat. Other species not tested.
- 宿主:
Mouse
- 规格:
100 ug
Primary antibody
纯化方法:
Purified
通用信息:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
产品描述:
This mAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
联系方式:
生物商城:http://www.annoron.com
办公电话:010-56256916
官方售后:400-965-8633
企业 QQ:3212874516、3198592576
地 址:北京经济技术开发区永昌中路4号院4号楼3层
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文献和实验The cytoplasm of nearly all eukaryotic cells contain mitochondria, although there is at least one exception, the protist Chaos (Pelomyxa) carolinensis . They are especially abundant in cells and parts of cells that are associated
线粒体 mitochondrion, pl.mitochondria
线粒体 mitochondrion, pl. mitochondria 除细菌和蓝藻中不存在之外,在所有动植物(真核生物)的细胞质中均可见到。它是宽约 0.5微米的线状和颗粒状的细胞器(希腊语 mito=线, chondr- on=粒),其主要功能为呼吸。每个细胞中线粒体的数目因不同细胞而异,在一个肝细胞中约有 2500个,一个植物细胞中有 100— 200个。线粒体含有高浓度的磷脂质和蛋白质,由于其折射率高于周围的细胞质基质,所以,即使在活细胞中用相差显微镜也能观察
the supernatant at 16,000 xg for 25 minutes at 4° C. Decant and discard the supernatant. Resuspend the pelleted mitochondria in 20 ml of 0.25 M sucrose in 10 mM HEPES. Skip to step 10. OR Optional: if cleaner mitochondria
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