产品名称:重组大鼠PDGF-BB
英文名称:Recombinant Rat Platelet-derived Growth Factor-BB
产品货号:GS4863
产品规格:2μg
分子量:Approximately 24.4kDa,a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide(B chain).
储存条件:冷冻(-20℃)
概述
Platelet-derived growth factor(PDGF)presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s.It is a disulfide-linked dimer consisting of two peptides-chain A and chain B.PDGF has three subforms:PDGF-AA,PDGF-BB,PDGF-AB.It is involved in a number of biological processes,including hyperplasia,embryonic neuron development,chemotaxis,and respiratory tubule epithelial cell development.The function of PDGF is mediated by two receptors(PDGFR-α and PDGFR-β).
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观
Sterile Filtered White lyophilized(freeze-dried)powder.
纯度
>98%by SDS-PAGE and HPLC analyses.
来源
Escherichia coli.
生物活性
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0ng/ml,corresponding to a specific activity of>5.0×105 IU/mg.
成分
Lyophilized from a 0.2μmfiltered concentrated solution in 1×PBS,pH 6.9.
内毒素
Less than 0.1 EU/μg of rRtPDGF-BB as determined by LAL method.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.