- ¥508
- Invitrogen™ eBioscience™
- 00-0044-59
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Invitrogen™ ELISA/ELISPOT Coating Buffer Powder 00-0044-59
- 供应商：
上海辅泽商贸有限公司
- 规格：
1 each
ELISA/ELISPOT Coating Buffer Powder
货号： 00-0044-59
| 货号 | 单位规格 | 单价 (CNY) | 数量 | |
|---|---|---|---|---|
| 00-0044-59 | 1 each | 508.00 |
规格
| Form: | Powder |
|---|---|
| Product Size: | 1 each |
| Reagent Type: | Coating Reagent |
| Validated Application: | ELISA, ELISPOT |
描述
The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.The eBioscience ELISA/ELISPOT Coating Buffer Powder is a dried powder formulation of phosphate buffered saline. Each packet is reconstituted in 1 Liter of distilled, deionized water, then filtered using a 0.22 um filter. The resulting 1x PBS (pH 7.4) is suitable for use as the ELISA/ELISPOT coating buffer.
Reported Application
ELISA, ELISPOT
For Research Use Only. Not for use in diagnostic procedures.
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文献和实验-Buffer® or LowCross-Buffer® STRONG as sample diluent, PlateBlock™ shows very good performance in serological ELISA tests, such as assays for neutralizing SARS-CoV-2 antibodies (s. also Fig. 5). The many benefits of animal-free solutions Animal
ELISPOT (Enzyme-linked ImmunoSPOT)
Assay Diluent (supplied as 5X): Dilute 5X solution to 1X in DI H2 O ELISPOT Wash Buffer: 1X PBS with 0.05% Tween-20 (0.5 ml Tween-20 in 1 L PBS) or eBioscience ELISA Wash Buffer Powder (Cat. No. 00-0400
。（1）本次洗涤目的在于：除去预湿用的乙醇。因此应该尽量洗涤干净，减少残留。（2）包被：按说明书操作。50μL/孔加入用1×PBS稀释好的包被抗体，4°C包被过夜。5、 封闭：（次日）倾倒包被液，用无菌1×PBS洗涤3次。最后一次，在灭菌的吸水纸上扣干。200μL/孔加入稀释好的封闭液，37°C封闭1h。6、倾倒封闭液，无须洗涤，直接加入检测细胞进行ELISPOT检测。二、Magi™ Coating Buffer润湿包被程序该方案中，用Magi™ Coating Buffer润湿PVDF孔后，直接加入
