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- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 适应物种:
Human
- 库存:
100
- 宿主:
Rabbit
- 应用范围:
IHC-P, E
- 靶点:
Amino acids 96 to 108 of human MAGEA12
- 规格:
50 μg
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文献和实验,可通过绘制标准曲线求出标本中E2F1 浓度。 试剂盒组成 ( 2-8 ℃保存) 酶标 板 (Coated Wells ) 96 孔 酶标抗体工作液(Enzyme Conjugate ) 12ml
Monoclonal Antibody Production Protocol
for 5 - 10 min. Aspirate the medium, resuspend pellet and wash again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free
monoclonal antibody production fusion
/ml (calculation figured out from the number of spleen cells the fusion was started from); let to stay in incubator, 37ѓC, pCO2 7% for 2 hours in a big flasc. add aminopterin; distribute on 96-wells cell culture plates (Nunc) (200µl
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