- ￥1375 - 5000
- Leading Biology
- APR04772G
- 美国
- 2025年10月04日
- WB, IF
- Rabbit
- M
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RBFOX1 Mouse Monoclonal Antibody [Clone ID: LBI4F9]
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
Pancreas/duodenum homeobox protein 1, Insulin promoter factor 1, IPF-1, Islet/duodenum homeobox 1, IDX-1, Somatostatin-transactivating factor 1, STF-1, Pdx1, Ipf1
- 浓度：
Affinity Purification
- 应用范围：
WB, IF
- 宿主：
Rabbit
- 适应物种：
M
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 克隆性：
Polyclonal
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 免疫原：
This Mouse Pdx1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 136-169 amino acids from the Central region of Mouse Pdx1.
- 规格：
400 µl/200 µl/100 µl/50 µl
| 规格： | 400 µl | 产品价格： | ￥5000.0 |
|---|---|---|---|
| 规格： | 200 µl | 产品价格： | ￥3250.0 |
| 规格： | 100 µl | 产品价格： | ￥2250.0 |
| 规格： | 50 µl | 产品价格： | ￥1375.0 |
细胞定位：Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:17052199}. Cytoplasm, cytosol
功能：Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
总结：Tissue Location: Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells)
稀释方法：IF--1:25 WB--1:1000
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文献和实验Double immunohistochemistry for BCL6 and PNA for Germinal Center detection
, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
chamber, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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