- ￥1375 - 5000
- Leading Biology
- APR04728G
- 美国
- 2025年09月29日
- WB
- Rabbit
- Rat
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SMAD2 Mouse Monoclonal Antibody [Clone ID: LBI4G7]
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
G1/S-specific cyclin-D3, Ccnd3
- 浓度：
Affinity Purification
- 应用范围：
WB
- 宿主：
Rabbit
- 适应物种：
Rat
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 克隆性：
Polyclonal
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 免疫原：
This rat Ccnd3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-66 amino acids from the N-terminal region of rat Ccnd3.
- 规格：
400 µl/200 µl/100 µl/50 µl
| 规格： | 400 µl | 产品价格： | ￥5000.0 |
|---|---|---|---|
| 规格： | 200 µl | 产品价格： | ￥3250.0 |
| 规格： | 100 µl | 产品价格： | ￥2250.0 |
| 规格： | 50 µl | 产品价格： | ￥1375.0 |
细胞定位：Nucleus {ECO:0000250|UniProtKB:P30281}. Cytoplasm {ECO:0000250|UniProtKB:P30281}
功能：Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4.
稀释方法：WB--1:500-1:1000
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Table I Species Protein A Protein G Human ++++ ++++ Mouse ++ ++ Rat
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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