- $395
- Leading Biology
- AMM01067G
- 2025年09月22日
- IHC, IF, FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG2a, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 库存：
100
- 宿主：
Mouse
- 应用范围：
IHC, IF, FC
- 规格：
50 ug
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文献和实验for 10 min at 400 x g to remove excess DETACHaBEAD. 14) Resuspend the cells in Buffer or Culture Medium. 15) The isolated cells are pure, viable and free from antibody bound to the surface, and may be used in any downstream application
with this for 30min to 1 hr. 5. Primary incubation. Make a solution of 1℃ antibody in blotto (or BSA/TBS/Tween). Need only ~5 mls or less for a modest size blot (14X8 cm). Antibody concentration should be optimized; a general guidline is ~1:2000 dilution for a red
Staining protocols for placental explant cultures
there is less likelihood of damaging the culture during processing. Incubate overnight (at least) at 4°C in blocking solution (4% BSA/PBS). Include 0.02% sodium azide if the plates are to be kept longer. Incubate in 1st antibody for 30 min at room temperature. Ideally
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