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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
Phosphatidylinositol 4-kinase type 2-beta, Phosphatidylinositol 4-kinase type II-beta, PI4KII-BETA, PI4K2B
- 浓度:
Affinity Purification
- 应用范围:
WB
- 宿主:
Rabbit
- 适应物种:
H, M
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 克隆性:
Polyclonal
- 保存条件:
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 形态:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- 免疫原:
This PI 4 Kinase type 2 beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-336 amino acids from the C-terminal region of human PI 4 Kinase type 2 beta.
- 规格:
400 µl/200 µl/100 µl/50 µl
| 规格: | 400 µl | 产品价格: | ¥5000.0 |
|---|---|---|---|
| 规格: | 200 µl | 产品价格: | ¥3250.0 |
| 规格: | 100 µl | 产品价格: | ¥2250.0 |
| 规格: | 50 µl | 产品价格: | ¥1375.0 |
细胞定位:Cytoplasm. Membrane. Note=Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism
功能:Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.
总结:Tissue Location: Widely expressed..
稀释方法:WB--1:2000
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文献和实验-3-3 associates with several tyrosine-phosphorylated proteins and phosphatidylinositol 3-kinase (PI3-K) in T cells. We report here the identification of the 120-kDa 14-3-3tau-binding phosphoprotein present in activated T cell lysates as Cbl, a protooncogene
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
are prepared using Adobe Photoshop and MicrosoftPowerpoint software. 2.5. Immunoprecipitation Kinase Activity Assays 1. The anti-GSK3b monoclonal antibody is from BD TransductionLaboratories (#610201) and protein G-Sepharose is fromAmersham (#17-0618-01
Introduction of human 14-3-3 proteins
with several tyrosine-phosphorylated proteins and phosphatidylinositol 3-kinase (PI3-K)in T cells.We report here the identification of the 120-kDa 14-3-3tau-binding phosphoprotein present in activated T cell lysates as Cbl,a protooncogene product of unknown function
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