Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
克隆性:
单克隆
适应物种:
Human
库存:
100
宿主:
Rabbit
应用范围:
WB, IHC
靶点:
A synthetic peptide corresponding to C-terminal residues of human TSC1/Hamartin was used as an immunogen.
抗体名:
KIAA0243, TSC
规格:
100 μl
分子量:
129767 Da
细胞定位:
Antigen Cellular Localization:Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes
形式:
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:
1. Lamb,et al. Nature Cell Biol 2:281-287
2. Manning BD, et al. Molec. Cell 10:151-162, 2002.
3. Astrinidis A, Senapedis W, et al. Hum Mol Genet 15:287-297, 2005.
产品概述:
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.This product is a high quality TSC1/Hamartin antibody.
功能:
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
总结:
Tuberous sclerosis complexes-1 (TSC1 also known as Hamartin) is a tumor suppressor that form a complex with TSC2 (Tuberin). This complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (2). Recently it has been shown that TSC1/Hamartin is localized to the centrosome and negatively regulates Plk1 implicating TSC1/hamartin to the regulation of centrosome duplication (3). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.
联系方式: