Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
克隆性:
单克隆
适应物种:
Human, Mouse, Rat
库存:
100
宿主:
Rabbit
应用范围:
WB
靶点:
A synthetic peptide corresponding to residues in the C-terminal of human eEF2 was used as an immunogen.
规格:
100 μl
分子量:
82144 Da
细胞定位:
形式:
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献:
1. Ryazanov et al. Proc Nat Acad Sci 94: 4884-4889, 1997
2. Ryazanov AG, et al. Nature 334:170-173, 1988.
3. Diggle, TA, et al. FEBS Lett 457:189-192, 1999.
4. Wang, XW, EMBO J 20:4370-4379, 2001.
5. End D, et al. J Biol Chem 257:9223-9225, 1988.
6. Severinov, KV, et al. New Biol 2: 887-893, 1990.
产品概述:
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.This product is a high quality eEF-2K antibody (C-term).
功能:
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
总结:
Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogenesis and malignant tumors (5-6).
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