- ￥100 - 500
- Affinity
- 美国
- AF2409
- 2025年07月06日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
保存于-20℃
- 克隆性：
单克隆
- 适应物种：
Human, Mouse, Rat
- 保质期：
可保存1-2年
- 抗原来源：
Rabbit
- 级别：
超纯
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 浓度：
1mg/ml
- 抗体英文名：
Phospho-Smad1 (Ser206) Antibody
- 抗体名：
Phospho-Smad1 (Ser206) Antibody
- 规格：
100ul
产品货号：AF2409
浓度：1 mg/ml
应用范围：WB, IHC, IF/ICC, ELISA
保质期：按说明书条件可保存1-2年
如何保存和分装抗体？
抗体保存应避免反复冻融，分装可以最大限度减少冻融对抗体造成的损坏，还可避免多次从同一管中移取抗体而引入污染。对于绝大多数抗体而言，分装为小等份在-20℃或-80℃下冻存是最佳的保存条件。但是每份不应该少于10 uL。分装的体积越小，抗体的 浓度受蒸发和管壁吸附的影响就会越大。大多数情况下，抗体在4 °C条件下能够保存1-2周。但是为了防止微生物污染，少量抗体可加入适当 浓度的NaN3。如果要用抗体对活细胞进行染色或处理，或者要用抗体进行体内研究，则不能使用含NaN3的抗体制备物；这种抗菌剂对大部分生物体都有毒害作用。部分加甘油的抗体，可直接储存在-20℃。
How do I store and aliquot antibodies?
Antibody storage should avoid repeated freeze-thaw cycles, as aliquoting minimizes damage to the antibody caused by freeze-thaw and avoids contamination by pipetting the antibody from the same tube multiple times. For the vast majority of antibodies, cryoppreservation at -20°C or -80°C in aliquots is optimal. However, each serving should not be less than 10 uL. The smaller the aliquot volume, the greater the influence of the antibody concentration on evaporation and wall adsorption. In most cases, antibodies can be stored at 4 °C for 1-2 weeks. However, in order to prevent microbial contamination, a small amount of antibody can be added at an appropriate concentration of NaN3. If you want to stain or treat live cells with antibodies, or if you want to use antibodies for in vivo studies, you can't use NaN3-containing antibody preparations; This antimicrobial agent has a toxic effect on most living organisms. Partially fortified glycerol antibodies can be stored directly at -20 °C.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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