For qPCR users using SYBR green dye who need to minimize optimization time, Applied Biosystems PowerUp SYBR Green Master Mix offers proprietary Dual-Lock hot start technology. Unlike other polymerases, a Dual-Lock polymerase offers two unique hot start mechanisms to prevent primer dimer and non-specific amplification.
PowerUp SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.
PowerUp SYBR Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments
The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 2 X 5 mL tube, sufficient for 1000 20-µL reactions (10 µL Master Mix per reaction).