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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Normal Human Mammary Epithelial Cells — Male
- 细胞类型:
人正常原代细胞
- 物种来源:
人源
- 器官来源:
人源
- 运输方式:
干冰或液氮
- 年限:
液氮储存10年以上
- 生长状态:
冻存
- 规格:
500,000 cells/vial
Lifeline® Normal Human Mammary Epithelial Cells (HMEC) – Male, when grown in Lifeline® MammaryLife™ Medium, provide an ideal serum-free culture model for the study of breast cancer development, three dimensional culture, carcinogen screening and other areas of breast research.
Lifeline® Male Mammary Cells have been isolated, plated, and expanded in culture vessels three times before being harvested for cryopreservation to ensure the highest viability and plating efficiency.
Our HMEC are quality tested in MammaryLife™ Medium to ensure optimal morphology and growth over a period of at least 15 population doublings. Lifeline HMEC are a mixed culture of epithelial cells bearing positive markers such as Cytokeratin 14 (myoepithelial), Cytokeratin 8/18 (luminal), and Cytokeratin 19 (luminal subpopulation).
500,000 cells per vial
Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline® Cell Technology provides products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.
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文献和实验实验材料:1. 人哺乳期早期或断奶后乳汁。2. 培养液:RPMI1640,15%FBS,10%人血清,50μg/ml霍乱毒素,0.5mg/ml氢化可的松,1mg/ml胰岛素。3. HuS(human serum):血库过期的血清,澳大利亚抗原阴性。5cm Nunc塑料培养皿。4. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素和200000U/L庆大霉素,pH7.4。5. 培养用液:1mg/ml胰岛素(Sigma):用6mmol/L盐
PriCells: 小鼠乳腺上皮细胞培养 实验材料: 1. 10-12周龄妊娠小鼠乳腺组织 2. 不含Ca 2+ 和Mg 2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素和200000U/L庆大霉素,pH7.4 3. FBS、含5%牛血清白蛋白(BSA)的PBS
厦门大学药物科学学院的研究团队已经发现表皮生长因子受体(EGFR)在三阴乳腺癌肿瘤位置高表达,并设计了靶向EGFR 的第三代 CAR 结构。随后用 EGFR CAR 慢病毒(EGFR CAR-T)感染原代T淋巴细胞生产 CAR-T,分别在体外和体内评估了 CAR-T 对 TNBC 的疗效。通过RNA测序分析揭示了 EGFR CAR-T 细胞中激活的信号通路情况。 结果发现:第三代 EGFR CAR-T 细胞在体外对 TNBC 肿瘤细胞有很强的特异性抑制作用,而对正常的乳腺上皮细胞或雌激素
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