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- lifeline
- 美国
- FC-0006
- 2026年03月30日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Human Umbilical Vein Endothelial Cells (HUVEC)，P2
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
Lifeline® normal Human Umbilical Vein Endothelial Cells (HUVEC), when grown in Lifeline® VascuLife® Medium, provide an ideal low serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology.
- Lifeline® HUVEC may be cultured with or without VEGF.
- HUVEC can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife® medium.
Lifeline® HUVEC are isolated from human umbilical cords, and cryopreserved as primary cells to ensure the highest viability and plating efficiency.
- HUVEC are isolated in VascuLife® EnGS Medium to ensure that they remain unexposed to recombinant human VEGF.
- Our HUVEC are quality tested in VascuLife® 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
- Lifeline® Umbilical Vein Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
500,000 cells per vial
Lifeline® umbilical vein endothelial cells have been quality tested for:
- Proliferation and Morphology: Normal cell appearance for 15 population doublings
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility: Negative for mycoplasma. Negative for bacterial and fungal growth
- Virus: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
- Specific Staining: von Willebrand Factor positive. Smooth muscle a-actin negative.
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文献和实验Isolation and Culture of Human Umbilical Vein Endothelial Cells
that control cell proliferation and modulate vessel wall tone (1 –3 ) Many of these, and other, processes can be studied in vitro using cultured cells, and umbilical veins are the most readily available source of human vascular endothelial cells. The method
Culture of Human Endothelial Cells from Umbilical Veins
of the isolation technique, expansion and maintenance under standard cell culture conditions. The human umbilical vein endothelial cell (HUVEC) model is useful for any research on general properties of human endothelium, but as these cells are of foetal and venous
iPS cells from human umbilical vein endothelial cells (HUVEC), an attractive source of the cells for reprogramming. Our method uses a multistep protocol in which reprogramming cells are selected by culturing in defined conditions on Matrigel
