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- 百奥莱博
- GS4323-FUZ
- 北京
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
329
- 英文名：
sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate
- 保质期：
长期
- 供应商：
北京百奥莱博科技有限公司
- 保存条件：
冷冻(-20℃)
- 规格：
50mg
特别提示：包括Sulfo-NHS-SS-Biotin在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
产品名称：Sulfo-NHS-SS-Biotin
英文名称：sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate
产品货号：GS4323
产品规格：50mg
分子量：606.7
储存条件：冷冻（-20℃）
概述
Sulfo-NHS-SS-Biotin(sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate)is a thiol-cleavable amine-reactive
biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding.
应用
This
reagent is water-soluble(to approximately 10mM)enabling biotinylation in the absence of organic solvents such as DMSO
or DMF.Water-solubility is advantageous for applications that cannot tolerate solvents or when inclusion of solvents
complicates the protocol.
The N-hydroxysulfosuccinimide(NHS)ester group on this reagent reacts with the ε-amine of lysine residues to produce a
stable product.Although α-amine groups present on the N-termini of peptides react with NHS esters,α-amines on proteins
are seldom accessible for conjugation.NHS esters react with primary amines in the deprotonated form and,therefore,the
reaction requires neutral to basic pH values to proceed.Primary amines react with NHS esters by nucleophilic attack and
Nhydroxysulfosuccinimideis released as a byproduct.Hydrolysis of the NHS-ester competes with the reaction in aqueous
solution.The rate of hydrolysis increases with increasing pH and occurs more readily in dilute protein solutions.
组份
50mg/bottle
技术规格
| 分子量 | 606.69 |
| 间隔臂 | 24.3? |
| 纯度 | ＞95%on TLC |
我公司销售的Sulfo-NHS-SS-Biotin报价，2-[生物素氨基]乙基-1,3-二硫基丙suān磺基琥珀酰亚胺酯质量上佳，价格普惠，欢迎广大新老客户踊跃订购。
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文献和实验Detection of Cell Surface Dopamine Receptors
and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surface. In the biotinylation method, cell surface receptors are labeled with Sulfo-NHS-biotin. The charge on the sulfonyl
Detection of Tight Junction Barrier Function In Vivo by Biotin
molecules with different sizes have been widely used to determine the TJ barrier function. In the in vivo system, the tracer molecule Sulfo-NHS-Biotin was initially used in Xenopus embryo system and subsequently was successfully applied to a number of animal
下面的操作方法可以使约3~5分子的生物素与1分子的蛋白结合，对某些蛋白来说，生物素与蛋白的分子比可根据不同的生物素化要求进行调整。 1. 溶解2~10 mg 蛋白于1 ml 的BuphTm磷酸盐缓冲液中，并计算溶解的毫摩尔数。如果蛋白含有不恰当的缓冲液（如Tris或者甘氨酸），可以通过在PBS中透析或浓缩的方法除去。计算：蛋白质量（mg）/蛋白分子量（MW）＝蛋白质的毫摩尔数。 2. 在打开之前，平衡生物素至室温。加2 mg Sulfo-NHS-Biotin于100 ul
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