Fevipiprant(QAW039) 使用注意事项 1. 微量试剂取用前请离心集液。 2. Annexin V-EGFP,Propidium Iodide(PI)避光保存及使用。 3. Propidium Iodide (PI)有毒,操作时请戴手套。 4. 本试剂盒适用于检测活细胞,流式细胞仪检测时,细胞数量不应低于1×105,,不推荐用于检测组织样本。 5. 推荐使用悬浮培养细胞。如果是贴壁细胞,需用不含EDTA的胰酶消化,如消化不当,可能引起假阳性,而用细胞刮子会造成细胞粘连成团,而影响检测。可将胰酶消化后细胞的保存在含2%BSA的PBS中,防止进一步的损伤。 6. 细胞固定后可能导致荧光的淬灭,请不要固定样品。 7. 因检测细胞的类型、凋亡诱导剂种类、使用的检测仪器不同,因而流式检测的荧光补偿也不同,因此建议每次检测均需使用未经凋亡诱导处理的细胞作为对照,进行荧光补偿的调节。 NCI-H446(人小细胞肺癌细胞) 5×106cells/瓶×2 COLO 205(结肠癌细胞) CL-0353hFOB 1.19(SV40转染人成骨细胞)5×106cells/瓶×2 KDR Others Mouse 小鼠 VEGFR2 / Flk-1 / CD309 / KDR 人细胞裂解液 (阳性对照) CAL 27人舌鳞癌细胞 Human tongue squamous cell carcinoma cell line CAL 27 DMEM+10% FBS IL36B Protein Human 重组人 IL36B / IL1F8 蛋白 小鼠肺大静脉平滑肌细胞完全培养基 100mL MCM7 微小染色体维持缺陷蛋白7抗体规格: 0.2ml MCSF 巨噬细胞克隆刺激因子抗体规格: 0.1ml Mcl1 髓样细胞白血病-1抗体规格: 0.2ml Phospho-Mcl1 (Ser159/Thr163) 磷酸化髓样细胞白血病-1抗体规格: 0.1ml MDM2 双微体2癌基因抗体规格: 0.1ml OrangeⅠ 橙黄Ⅰ 质量规格:>98.0%(HPLC)(T) PatentBlueVF 专利蓝VF 质量规格:>98.0%(HPLC)(T) Phenolphthalein 酚酞(IND) 质量规格:>98.0%(HPLC)(T) Potassiumiodidesolution 碘化钾溶液(100g/L) 质量规格:>98.0%(HPLC)(T) Potassiumiodidesolution 碘化钾溶液(1/60mol/L(0.1N)) 质量规格:>98.0%(HPLC)(T) Rosolicacid 玫红酸(AR) 质量规格:>98.0%(HPLC)(T) Rosolicacid 玫红酸(指示剂级) 质量规格:>98.0%(HPLC)(T) Sodiumoxalatesolutionforvolumetricanalysis 草酸钠容量分析用溶液标准物质 质量规格:>98.0%(HPLC)(T) Thymolblue 百里香酚蓝(IND) 质量规格:>98.0%(HPLC)(T) Thymolblue 百里香酚蓝(ACSreagent,95%) 质量规格:>98.0%(HPLC)(T) QuinaldineRed 喹哪啶红 质量规格:>98.0%(HPLC)(T) Phenolphthalein 酚酞(98%) 质量规格:>98.0%(HPLC)(T) Tetrabromophenolblue 四溴酚蓝 质量规格:>98.0%(HPLC)(T) Thymolphthalein 百里香酚酞 质量规格:>98.0%(HPLC)(T) Thorin 钍试剂 质量规格:>98.0%(HPLC)(T) Fevipiprant(QAW039) 中文名称 精子生成相关蛋白3抗体 别 名 1700011N12Rik; 1700029H01Rik; 4930424D10Rik; Mtsarg1; SPATA3; Spermatogenesis-associated protein 3; SPTA3_HUMAN; Testis and spermatogenesis cell-related protein 1; Testis spermatocyte apoptosis-related protein 1; Tsarg1. 浓 度 1mg/1ml 规 格 0.2ml/200μg 抗体来源 Rabbit 克隆类型 polyclonal 交叉反应 Human, Mouse, Rat, Rabbit 产品类型 一抗 研究领域 细胞生物 发育生物学 干细胞 性 状 Lyophilized or Liquid 免 疫 原 KLH conjugated synthetic peptide derived from human SPATA3/Tsarg1 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 产品介绍 TSARG1 is a 183 amino acid protein predominantly expressed in the testis. TSARG1 was initially discovered due to its possible role in spermatogenesis or spermatogenesis cell apoptosis and it may be linked to cell cycle control. Apoptosis during spermatogenesis is a natural occurrence and typically effects 75-80% of the germ cells. This high rate of apoptosis is required for normal mature speramatogenesis. It may be a consequence of mutated DNA but it is also believed to ensure a proper ratio between Sertoli cells and germ cells.