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- 文献和实验
- 技术资料
- 库存:
33
- 英文名:
Total Antioxidant Capacity Assay Kit with FRAP method
- 保质期:
详见说明书
- 供应商:
上海一研
- 保存条件:
低温
- 规格:
100次
英文名称:Total Antioxidant Capacity Assay Kit with FRAP method
产品规格:100次
发货周期:1~3天
本试剂盒是一种采用Ferric Reducing Ability of Plasma(FRAP)方法,可以对血浆、血清、唾液、尿液等各种体液,细胞或组织等裂解液、植物或中草药抽提液、或各种抗氧化物溶液的总抗氧化能力进行检测的试剂盒。本试剂盒可以检测100个样品。
试剂盒组份:
TPTZ稀释液——————15ml
TPTZ溶液———————1.5ml
检测缓冲液——————1.5ml
FeSO4?7H2O——————200mg
Trolox溶液 (10mM)———0.1ml
活性氧ROS主要包括羟基自由基、超氧自由基和过氧化氢。在细胞或组织的正常生理代谢过程中会产生活性氧,同时一些环境因子例如紫外照射、γ射线照射、吸烟、环境污染等也可以诱导活性氧的产生。活性氧产生后,可以导致细胞内脂、蛋白和DNA等的氧化损伤,诱发氧化应激(Oxidative stress),继而导致各种肿瘤、动脉粥样硬化、风湿性关节炎、糖尿病、肝损伤、以及中枢神经系统疾病等。
机体中存在多种抗氧化物,包括抗氧化大分子、抗氧化小分子和酶等,可以清除体内产生的各种活性氧,以阻止活性氧诱导的氧化应激(oxidative stress)的产生。一个体系内的各种抗氧化大分子、抗氧化小分子和酶的总的水平即体现了该体系内的总抗氧化能力。因此测定血浆、血清、尿液、唾液等各种体液,细胞或组织等裂解液中的总抗氧化能力具有非常重要的生物学意义。
植物或中草药抽提液、或各种抗氧化物溶液的总抗氧化能力的检测可以用于检测各种溶液的抗氧化能力的强弱,可以用于筛选强抗氧化能力的药物。
储存条件:-20℃,有效期12个月。
总抗氧化能力检测试剂盒(FRAP法) 使用注意事项
1. 微量试剂取用前请离心集液。
2. Annexin V-EGFP,Propidium Iodide(PI)避光保存及使用。
3. Propidium Iodide (PI)有毒,操作时请戴手套。
4. 本试剂盒适用于检测活细胞,流式细胞仪检测时,细胞数量不应低于1×105,,不推荐用于检测组织样本。
5. 推荐使用悬浮培养细胞。如果是贴壁细胞,需用不含EDTA的胰酶消化,如消化不当,可能引起假阳性,而用细胞刮子会造成细胞粘连成团,而影响检测。可将胰酶消化后细胞的保存在含2%BSA的PBS中,防止进一步的损伤。
6. 细胞固定后可能导致荧光的淬灭,请不要固定样品。
7. 因检测细胞的类型、凋亡诱导剂种类、使用的检测仪器不同,因而流式检测的荧光补偿也不同,因此建议每次检测均需使用未经凋亡诱导处理的细胞作为对照,进行荧光补偿的调节。
Arthrinium spp.节菱孢霉属通用PCR试剂盒别 名 Abhd1; ABHD1_HUMAN; Abhydrolase domain containing 1; Abhydrolase domain-containing protein 1; Alpha/beta hydrolase domain containing protein 1; FLJ36128; LABH1; Lung alpha/beta hydrolase 1; Lung alpha/beta hydrolase protein 1.免 疫 原 KLH conjugated synthetic peptide derived from human ABHD1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrinium spp.节菱孢霉属通用染料法荧光定量PCR试剂盒别 名 ABHD13; ABHDD_HUMAN; abhydrolase domain containing 13; Abhydrolase domain-containing protein 13; BEM46L1; C13orf6.免 疫 原 KLH conjugated synthetic peptide derived from human ABHD13产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrinium spp.节菱孢霉属通用探针法荧光定量PCR试剂盒别 名 Abhydrolase domain containing 14B; Abhydrolase domain containing protein 14B; CCG1 interacting factor B; Cell cycle gene 1 interacting factor B; CIB; MGC15429; OTTHUMP00000212469; ABHEB_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ABHD14B产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrobacter equi马节杆菌PCR试剂盒别 名 ABH15_HUMAN; Abhd15; Abhydrolase domain containing protein 15; Abhydrolase domain-containing protein 15; hypothetical protein LOC116236; LOC116236; RGD1304598.免 疫 原 KLH conjugated synthetic peptide derived from human ABHD15产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrobacter equi马节杆菌染料法荧光定量PCR试剂盒别 名 ABHD3; Abhydrolase domain containing 3; Alpha/beta hydrolase domain containing protein 3; LABH3; Lung alpha/beta hydrolase 3; MGC11259; ABHD3_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ABHD3产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrobacter equi马节杆菌探针法荧光定量PCR试剂盒别 名 ATP binding cassette sub family F (GCN20) member 3; ATP binding cassette sub family F member 3; EST201864; FLJ11198; ABCF3_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ABCF3产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrobacter spp.节杆菌通用PCR试剂盒别 名 ABC13; ATP binding cassette sub family A (ABC1) member 5; ATP binding cassette A5; ATP binding cassette sub family A member 5; DKFZp451F117; DKFZp779N2435; EST90625; FLJ16381; KIAA1888; ABCA5_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ABCA5产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrobacter spp.节杆菌通用染料法荧光定量PCR试剂盒别 名 aarF domain containing kinase 1; ADCK 1; BOS_11212; FLJ39600; MGC142877; Uncharacterized aarF domain containing protein kinase 1; 2610005A10Rik; ADCK1_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ADCK1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Arthrobacter spp.节杆菌通用探针法荧光定量PCR试剂盒别 名 ACBD 4; Acyl CoA binding domain containing protein 4; Acyl coenzyme A binding domain containing 4; ACBD4_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ACBD4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Ascosphaera apis蜜蜂球囊菌PCR试剂盒别 名 ABH4; Abhd4; ABHD4_HUMAN; Abhydrolase domain containing 4; Abhydrolase domain-containing protein 4; Alpha/beta hydrolase 4; Alpha/beta-hydrolase 4; FLJ12816; Lyso N acylphosphatidylethanolamine lipase; Lyso-N-acylphosphatidylethanolamine lipase.免 疫 原 KLH conjugated synthetic peptide derived from human ABHD4产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Ascosphaera apis蜜蜂球囊菌染料法荧光定量PCR试剂盒别 名 Aldehyde dehydrogenase 16 family, member A1; Aldehyde dehydrogenase family 16 member A1; MGC10204; A16A1_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ALDH16A1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Ascosphaera apis蜜蜂球囊菌探针法荧光定量PCR试剂盒别 名 Adhesion molecule with Ig like domain 3; ALI3; Alivin 3; AMIGO 3; Amphoterin induced gene and ORF 3; Amphoterin induced protein 3 precursor.AMGO3_HUMAN免 疫 原 KLH conjugated synthetic peptide derived from human AMIGO3产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Aspergillus flavus黄曲霉PCR试剂盒别 名 AK1A1_HUMAN; Akr1a1; Alcohol dehydrogenase [NADP+]; Alcohol dehydrogenase; Aldehyde reductase; aldo-keto reductase family 1 member A1 (aldehyde reductase); Aldo-keto reductase family 1 member A1; ALDR1; ALR.免 疫 原 KLH conjugated synthetic peptide derived from human AKR1A1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Aspergillus flavus黄曲霉染料法荧光定量PCR试剂盒别 名 ALAD; ALADH; ALADR; Aminolevulinate dehydratase; Aminolevulinate, delta, dehydratase; Delta aminolevulinic acid dehydratase; Delta-aminolevulinic acid dehydratase; HEM2_HUMAN; Lv; PBGS; Porphobilinogen synthase.免 疫 原 KLH conjugated synthetic peptide derived from human ALAD产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Aspergillus flavus黄曲霉探针法荧光定量PCR试剂盒别 名 ALS2CR4 protein, N terminus truncated; Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 4; TM237_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human ALS2CR4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
YAP1 原癌基因Yes相关蛋白1抗体Rhesus antibody Rh规格 0.2ml
XTP4 乙型肝炎病毒X蛋白反转录蛋白4抗体Rhesus antibody Rh规格 0.2ml
XRCC4 X射线修复交叉互补蛋白4抗体Rhesus antibody Rh规格 0.2ml
XRCC3 X射线修复交叉互补蛋白3抗体Rhesus antibody Rh规格 0.2ml
XRCC1 X射线修复交叉互补蛋白抗体Rhesus antibody Rh规格 0.1ml
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髓过氧化物酶特性抗中性粒细胞胞质检测试剂盒对照品进口/分装聚二醇3350标准品聚二醇3350标准品48T/96T2ml(外)HTB-122
Penicillin G 青霉素G抗体进口/分装小鼠E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA Kit,48T/96T
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8-OHdG 8-羟基脱氧鸟苷抗体特价促销大鼠E钙黏蛋白/上皮钙黏蛋白(E-Cad)ELISA Kit,48T/96T
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文献和实验小鼠总抗氧化能力的测定 刘小美 宋菊敏 (2006-10-24) 一、原理 机体中有许多抗氧化物质,能使Fe3 + 还原成Fe2 + ,后者可与菲啉类物质形成稳固的络合物,通过比色可测出其抗氧化能力的高低。 二、目的 1.掌握总抗氧化能力
摘要 : 目的: 探讨一氧化氮(nitric oxide , NO) 、总抗氧化能力(total antioxide capacity , T2AOC) 对大鼠隐睾生殖细胞凋亡的影响。 方法: 健康SD 雄性大鼠20 只,日龄22 d 时复制单侧隐睾模型。生物素2dUTP/ 酶标亲和素测定法检测睾丸生殖细胞凋亡,采用硝酸还原酶法测定睾丸组织中NO 含量,化学比色法测定睾丸组织中T2AOC
FRAP Analysis of Chemosensory Components of Dictyostelium
after photobleaching (FRAP), has been used in Dictyostelium to investigate a number of cellular processes including actin and cytoskeleton dynamics during chemotaxis and cytokinesis (J. Muscle Res. Cell Motil. 23:639–649, 2002; Biophys. J. 81:2010–2019, 2001; Mol. Biol
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