Exo-Fect™ siRNA/miRNA Transfection Kit
产品名称:Exo-Fect siRNA/miRNA Transfection Kit
规 格:20 Reactions
货 号: EXFT200A-1
厂 家: SBI
概述:
Headache-free loading of siRNAs and miRNAs into extracellular vesicles
Whether you’re engineering extracellular vesicles (EVs) as part of therapeutics development1 or using them as a tool for functional studies of EV-mediated nucleic acid delivery2, getting the desired miRNA or siRNA cargo into EVs is a critical first step. SBI helps you efficiently and effortlessly get this step done with the Exo-Fect™ siRNA/miRNA Transfection Kit, a first-in-class reagent specifically designed for loading small RNAs into EVs.
Unlike electroporation, a common method for introducing small RNAs into EVs3, the Exo-Fect workflow does not require access to specialized equipment or the same time-consuming optimization of voltage and timing. Instead, it leverages our proprietary Cell-Penetrating Peptide (CPP) technology to transfer siRNAs or miRNAs to the inside of your EVs.
This next-generation EV transfection reagent offers the following benefits:
1,Efficient delivery—siRNA/miRNA-loaded EVs deliver cargo to as many as 95% of target cells*
2,Validated efficacy—siRNA-loaded EVs lead to significant knockdown (>80%) of target gene expression in transfected cells**
3,No toxicity—unlike commonly-used lipid-based transfection reagents, the Exo-Fect siRNA/miRNA Transfection Reagent causes no toxicity in tested cells
4,Fast, easy workflow—go from prepared small RNA to ready-to-use, RNA-loaded EVs in less than 1.5-hours and with only 15-minutes of hands-on time
5,Minimal background—a clean-up step ensures complete removal of free small RNA and excess transfection reagent for clean, ready-to-use, RNA-loaded EVs
*As assessed by exposing non-fluorescent target cells to EVs loaded with Cy3-labeled control siRNA using Exo-Fect siRNA/miRNA transfection reagent, and then measuring the percentage of fluorescent cells (HeLa, HUVEC, and HEK293 cells were all tested).
** As assessed using qPCR analysis of HPRT gene expression in cells treated with either HPRT-targeting siRNA or control siRNA.
操作流程:
Quick and easy loading of siRNA or miRNA into EVs
The Exo-Fect siRNA/miRNA Transfection Kit workflow is quick and easy—complete in 1.5 hours and with only 15-minutes of hands-on time.
The workflow consists of two steps:
Load your siRNA or miRNA into already-isolated EVs.
Incubate siRNA or miRNA with Exo-Fect siRNA/miRNA Transfection reagent for 15-minutes at room temperature.
Add isolated EVs to the mixture and incubate at 37°C for 1-hour.
Clean-up the reaction by removing free siRNA/miRNA, transfection reagent, and free siRNA/miRNA-Exo-Fect complexes
Transfer reaction to the included, pre-washed spin-column and incubate with gentle rotation for 10-minutes at room temperature.
Collect EVs by centrifuging the spin-column for 30-seconds at 1,000 x g. Loaded EVs are ready for use in downstream applications.