产品货号:jlcR18525 英文名称 :Nrif1 中文名称 :神经营养因子受体相互作用因子1抗体 别 名 :Neurotrophin receptor-interacting factor 1; receptor-interacting factor; Nrif1; Nrif; NRIF1_MOUSE; Zinc finger protein 110; Zfp110. 研究领域 :转录调节因子 锌指蛋白 表观遗传学 抗体来源 :Rabbit 克隆类型 :Polyclonal 交叉反应 :Mouse, Rat, 产品应用 :ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 :94kDa 细胞定位 :细胞核 性 状 :Lyophilized or Liquid 浓 度 :1mg/ml 免 疫 原 :KLH conjugated synthetic peptide derived from mouse Nrif1:701-800/828 亚 型 :IgG 纯化方法 :affinity purified by Protein A 储 存 液 :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 :Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed :PubMed 产品介绍 : Zfp110 (zinc finger protein 110) contains 1 SCAN box domain. The SCAN domain has been shown to be able to mediate homo- and hetero-oligomerisation.
Function: Transcription regulator involved in NGFR/p75(NTR)-mediated apoptosis. Essential component of the NGFR/p75(NTR) apoptotic pathway: upon ligand-binding and subsequent cleavage of NGFR/p75(NTR), binds to the intracellular domain (ICD) cleavage product of NGFR/p75(NTR), translocates to the nucleus and induces apoptosis, possibly by regulating expression of key regulators of apoptosis. Induces NGFR/p75(NTR)-mediated apoptosis in retina and sympathetic neurons. May also regulate expression of neuronal cholesterol biosynthesis genes. Probably acts as a transcription repressor: specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin-modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression.
Subunit: Interacts with NGFR/p75(NTR). Interacts (via KRAB 1 domain) with TRAF6. Interacts (when ubiquitinated at Lys-15) with SQSTM1/p62.
Tissue Specificity: Ubiquitously expressed at low level. Expressed at higher level in testis.
Post-translational modifications: Ubiquitinated by TRAF6 at Lys-15 through 'Lys-63'-linked polyubiquitination. 'Lys-63'-linked polyubiquitination occurs in response to NGFR/p75(NTR) cleavage by gamma-secretase and promotes binding with the ICD cleavage product of NGFR/p75(NTR), followed by tranlocation into the nucleus and subsequent apoptosis.
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. Contains 2 KRAB domains. Contains 1 SCAN box domain.
SWISS: Q923B3
Gene ID: 65020
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.