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文献和实验成分 硫乙醇酸钠 1.5g 磷酸氢二钠 1.1g 氯化钠 5g 琼脂 5g 蒸馏水 1000mL 1%氯化钙溶液 9mL pH8.4 制法 除氯化钙外,其他均按上述成分配制,加热溶解。冷至50℃,加入氯化钙溶液,校正pH到8.4。分 装试管,每支5mL,或注入具有胶塞的瓶内。121℃高压灭菌15min。 用途 作空肠
Synchronizing Protein Transport in the Secretory Pathway
?intersecting and non?intersecting pathways exist in cells to carry out proper transport, modification, and addressing. To analyze and visualize the intracellular trafficking of any secretory protein, we developed the retention using selective hooks (RUSH
Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport
Ca2+ -sensitive electrode as a practical approach is used to follow Ca2+ changes in the medium and particularly useful to study mitochondrial Ca2+ uptake (or release); this method permits the continuous recording of Ca2+ movements
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