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9cm x 10皿
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文献和实验:dextrose、L-arginine HCl、Difco PPLO broth (Difco 0554-17-1) horse serum、15% yeast extract solution (autoclaved)、Bacto agar、无菌有盖螺旋试管(autoclaved)、无菌培养皿﹙60x15mm﹚、L-玻棒、37℃培养箱、厌氧缸(厌氧缸长期培养易有污染,所以厌氧包应用无菌水,每次打开厌氧缸后,用70% ethanol擦拭内壁。) · 培养基制备:基本配方为Difco PPLO broth
in your cell culture rooms and even within your hoods(it should be 0 in hoods) by using Nutrient Agar and Sabouraud Dextrose Agar plates. Nutrient agar will give you information about airborne bacteria levels and Sabouraud dextrose agar will detect airborne
of denaturing solution (x1) and neutralizing solution (x2) for each filter at 6" intervals on Saran wrap. Using forceps, carefully peel nylon membrane from agar surface and place it colony side up onto a puddle of denaturing solution. The colonies
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