Recombinant Human VEGF 165 Pro

Recombinant Human VEGF 165 Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED₅₀ for this effect is 1-6 ng/mL.
The specific activity of recombinant human VEGF₁₆₅ is approximately 1.7 x 10³ U/μg, which is calibrated against recombinant human VEGF₁₆₅ WHO Standard (NIBSC code: 02/286).

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein
Ala27-Arg191
Accession #
NP_001165097
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
19.2 kDa (monomer)
SDS-PAGE
20-22 kDa, reducing conditions
39-42 kDa, non-reducing conditions