重组eGFP北京品牌

特别提示：包括重组eGFP在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组eGFP
英文名称：Recombinant Enhance Green Fluorescent Protein
产品货号：GS4653
产品规格：10μg

分子量：Approximately 26.9kDa,a single non-glycosylated polypeptide chain containing 239 amino acids.
储存条件：冷冻（-20℃）

概述

Green fluorescent protein(GFP)here refers to the protein first purified from jellyfish Aequorea victoria,though many other organisms have similar proteins.It is a 26.9 kDa protein(composed of 238 a.a.residues)that shows green fluorescence in short-wave light(blue to ultraviolet).Despite of wild-type GFP,many mutants of GFP have been engineered for wider usage in research.Enhanced GFP(eGFP)has S65T and F64Lmutations,which make GFP show increased fluorescence and fold more efficiently under 37℃,respectively.eGFP allows the use of GFP in mammalian cells.In A.Victoria,GFP plays roles as an energy transfer acceptor.It has long been used in cell and molecular biology as a reporter of gene expression.GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of reGFP as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

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