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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
江西江蓝纯生物试剂有限公司
- 库存:
81
- 英文名:
Rosetta感受态细胞
菌株类型: E.coli
培养基: LB培养基
生长条件: 37 ℃, 有氧
基因型: Rosetta感受态细胞基因型
抗 性: 氯霉素
质粒转化条件: 42 ℃热激
应 用: 蛋白表达
诱导方法: IPTG
Rosetta感受态细胞
菌株特点:
Rosetta系列菌株来源于BL21系列宿主菌,该系列菌株含有原本在大肠杆菌中稀少的真核细胞密码子,增加了真核细胞的蛋白表达水平。
Rosetta(DE3)菌株含有pRARE质粒,能够提供AUA, AGG, AGA, CUA, CCC, 和GGA稀有密码子的tRNA,该质粒具有氯霉素抗性。
Rosetta(DE3)载体通过提供稀有密码子,使得该宿主菌相对于其他大肠杆菌,能够提供更加“通用”的蛋白质表达,从而提升目的蛋白表达水平。
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文献和实验proteins, termed Rosetta stone sequences, help link disparate proteins together, and suggest the likelihood of functional interactions between the linked entities, describing local and global relationships within the proteome. These relationships help
Redesigning the Specificity of ProteinDNA Interactions with Rosetta
, we present the current state of protein–DNA interface design with the Rosetta macromolecular modeling program. The LAGLIDADG endonuclease family of DNA-cleaving enzymes, under study as potential gene therapy reagents, has been the main testing ground
Rosetta Ligand Docking with Flexible XML Protocols
[1]. The latest release of Rosetta ligand software includes many new features, such as (1) docking of multiple ligands simultaneously, (2) representing ligands as fragments for greater flexibility, (3) redesign of the interface during docking, and (4) an XML
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