细胞名称: PLC/PRF/5人肝癌压力山大细胞 种属来源: 人
组织来源: 肝脏,亚力山大细胞
疾病特征: 肝癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: MEM培养基),90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 2
STR: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 15,16
备 注: Nucleotide (GenBank) : X75311 H.sapiens /Hepatitis B virus fusion mRNA for mevalonate kinase.
Nucleotide (GenBank) : X77700 H.sapiens / Hepatitis B virus (A4.0') DNA for mevalonate kinase (partial).
参考文献: Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321
MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208
Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138
Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960
Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551
Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281