细胞名称: MDA-MB-468人乳腺癌细胞 种属来源: 人
组织来源: 乳房,乳腺
疾病特征: 乳腺癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: L-15培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
应 用: 该MDA-MB-468人乳腺癌细胞可以作为转染宿主细胞。
STR: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 8
THO1: 7
TPOX: 8,9
vWA: 18
同工酶: AK-1, 1
ES-D, 1
G6PD, A
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 2
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Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262
Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845
Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006
Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591