细胞名称: ATCC TIB-68(WEHI-3)小鼠白血病细胞 种属来源: 小鼠
组织来源: 外周血
疾病特征: 白血病
细胞形态: 圆形细胞样
生长特性: 贴壁生长
培 养 基: DMEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
应 用: 该ATCC TIB-68(WEHI-3)小鼠白血病细胞在抗体依赖性细胞介导的细胞毒性作用中表现出弱的效应。
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Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922
McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720