细胞名称: ATCC HTB-181(NCI-H820)人乳头状腺癌肺癌 种属来源: 人
组织来源: 肺
疾病特征: 乳头状腺癌肺癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: DMEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
STR: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 9,11
D7S820: 10,13
THO1: 8
TPOX: 12
vWA: 18
同工酶: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
参考文献: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953
The NCI-H820 cell line was derived by A.F. Gazdar and H. Oie in 1984 from the lymph node of a patient with papillary adenocarcinoma of the lung taken prior to treatment.
The cells form typical papillary adenomas.