细胞名称: ATCC HTB-67(SK-MEL-1)人皮肤黑色素瘤细胞 种属来源: 人
组织来源: 皮肤
疾病特征: 皮肤黑色素瘤
细胞形态: 球形细胞样
生长特性: 悬浮生长
培 养 基: MEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
STR: Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 12
THO1: 6
TPOX: 11
vWA: 16,17
同工酶: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1
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Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447