细胞名称: ATCC HTB-1(J82)人膀胱癌细胞 种属来源: 人
组织来源: 膀胱
疾病特征: 移行细胞癌
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: MEM培养基,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
STR: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 10,12
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 9.3
TPOX: 11,12
vWA: 17,18
同工酶: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1-2
PGM1, 1
PGM3, 2
参考文献: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
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Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484