细胞名称: ATCC HB-198(F4/80)小鼠杂交瘤细胞 种属来源: 小鼠
组织来源: 杂交瘤
疾病特征: 杂交瘤
细胞形态: 淋巴母细胞样
生长特性: 悬浮生长
培 养 基: RPMI 1640,90%;FBS,10%。
生长条件: 气 相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
参考文献: Hume DA, et al. The mononuclear phagocyte system of the mouse defined by immunohist ochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med. 158: 1522-1536, 1983. PubMed: 6355361
Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 1981. PubMed: 7308288