细胞名称: ATCC CRL-5803(NCl-H1299)人非小细胞肺癌细胞 种属来源: 人
组织来源: 肺
疾病特征: 肺癌;非小细胞肺癌;转移灶;淋巴结
细胞形态: 上皮细胞样
生长特性: 贴壁生长
培 养 基: RPMI-1640,90%;FBS,10%。
生长条件: 气相:空气,95%;二氧化碳,5%; 温度:37 ℃,
传代方法: 1:2至1:6,每周2次。
冻存条件: 90% 完全培养基+10% DMSO,液氮储存
支原体检测: 阴性
安全等级: 1
应 用: 该细胞可以作为转染宿主细胞。
STR:
Amelogenin:X
CSF1PO:12
D13S317:12
D16S539:12,13
D5S818:11
D7S820:10
THO1:6,9.3
TPOX:8
vWA: 16,17,18
参考文献: Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy.